Descripciones mejoradas de Syndetics:

Many of the available books in cellular and molecular biology fail to explain the principles and methods described. Handbook of Molecular and Cellular Methods in Biology and Medicine fills in these gaps. Not only do the authors describe the many methods and techniques most commonly used, but they also discuss why you would use each particular method.

This handbook covers a wide range of techniques and methods that have been developed and extensively tested. Described in a logical framework of 21 chapters, methods include isolation and purification of DNA, RNA, and proteins; preparation of hybridization probes; electrophoresis and blotting techniques; construction of cDNA and genomic DNA libraries; DNA sequencing; PCR technology; DNA recombination and mutagenesis; transcription in vitro and translation in vitro; gene transfer and analysis of gene expression in transgenic animals and plants; cell and tissue cultures; and microscopy, as well as bioseparation techniques and preparation of monoclonal and polyclonal antibodies.

The authors provide descriptive illustrations for each method. Step-by-step instructions allow you to use this book in the lab while performing the various techniques. Each chapter provides the principles behind the method, easy-to-follow instructions, the reagents and materials required, and references for further study. Many chapters also include a troubleshooting guide, cautioning what to avoid and indicating steps that require extra care. Every biologist needs this book on their lab bench!

Tabla de contenidos provista por Syndetics

• Chapter 1. Isolation and Purification of DNA(p. 1)
• Chapter 2. Isolation and Purification of RNA(p. 25)
• Chapter 3. Extraction and Purification of Proteins(p. 45)
• Chapter 4. Preparation of Nucleic Acid Probes(p. 67)
• Chapter 5. Southern Blot Hybridization(p. 85)
• Chapter 6. Northern Blot Hybridization(p. 105)
• Chapter 7. Preparation of Monoclonal and Polyclonal Antibodies against Specific Protein(s)(p. 113)
• Chapter 8. Western Blot Hybridization(p. 127)
• Chapter 9. cDNA Libraries(p. 147)
• Chapter 10. Genomic DNA Libraries(p. 193)
• Chapter 11. DNA Sequencing and Analysis(p. 237)
• Chapter 12. DNA Site-Directed and Deletion Mutagenesis(p. 271)
• Chapter 13. DNA Footprinting and Gel-Retardation Assay(p. 291)
• Chapter 14. Differential Display(p. 305)
• Chapter 15. Functional Genomics and DNA Microarray Technology(p. 319)
• Chapter 16. In Vitro Translation of mRNA(s) and Analysis of Protein by Gel Electrophoresis(p. 347)
• Chapter 17. Plant Tissue and Cell Culture(p. 357)
• Chapter 18. Gene Transfer and Expression in Animals(p. 377)
• Chapter 19. Gene Transfer and Expression in Plants(p. 409)
• Chapter 20. Inhibition of Gene Expression(p. 433)
• Chapter 21. Microscopy: Light, Scanning Electron, Environmental Scanning Electron, Transmission Electron, and Confocal(p. 461)
• Chapter 22. Localization of Gene Expression(p. 487)
• Chapter 23. Bioseparation Techniques and Their Applications(p. 509)
• Chapter 24. Combinatorial Techniques(p. 535)
• Chapter 25. Computational Data-Mining Methods Applied to Combinatorial Chemistry Libraries(p. 549)
• Index(p. 565)