Tabla de contenidos provista por Syndetics

• Preface(p. v)
• Contributors(p. xiii)
• Part I Introduction to PCR
• 1 A Short History of the Polymerase Chain Reaction(p. 3)
• 2 PCR Patent Issues(p. 7)
• 3 Equipping and Establishing a PCR Laboratory(p. 15)
• 4 Quality Control in PCR(p. 21)
• Part II Preparation of Nucleic Acid Templates
• 5 Extraction of Nucleic Acid Templates(p. 27)
• 6 Extraction of DNA from Whole Blood(p. 29)
• 7 DNA Extraction from Tissue(p. 33)
• 8 Extraction of DNA from Microdissected Archival Tissues(p. 35)
• 9 RNA Extraction from Blood(p. 43)
• 10 RNA Extraction from Frozen Tissue(p. 45)
• 11 RNA Extraction from Tissue Sections(p. 47)
• 12 Dual DNA/RNA Extraction(p. 49)
• 13 DNA Extraction from Fungi, Yeast, and Bacteria(p. 53)
• 14 Isolation of RNA Viruses from Biological Materials(p. 55)
• 15 Extraction of Ancient DNA(p. 57)
• 16 DNA Extraction from Plasma and Serum(p. 63)
• 17 Technical Notes for the Detection of Nucleic Acids(p. 65)
• 18 Technical Notes for the Recovery and Purification of PCR Products from Acrylamide Gels(p. 77)
• Part III Basic PCR Methods
• 19 PCR Primer Design(p. 81)
• 20 Optimization of Polymerase Chain Reactions(p. 89)
• 21 Subcycling PCR for Long-Distance Amplifications of Regions with High and Low Guanine-Cystine Content: Amplification of the Intron 22 Inversion of the FVIII Gene(p. 101)
• 22 Rapid Amplification of cDNA Ends(p. 105)
• 23 Randomly Amplified Polymorphic DNA Fingerprinting: The Basics(p. 117)
• 24 Microsphere-Based Single Nucleotide Polymorphism Genotyping(p. 123)
• 25 Ligase Chain Reaction(p. 135)
• 26 Nested RT-PCR in a Single Closed Tube(p. 151)
• 27 Direct PCR from Serum: Application to Viral Genome Detection(p. 161)
• 28 Long PCR Amplification of Large Fragments of Viral Genomes: A Technical Overview(p. 167)
• 29 Long PCR Methodology(p. 173)
• Part IV Ultrasensitive and Quantitative PCR
• 30 Qualitative and Quantitative PCR: A Technical Overview(p. 181)
• 31 Ultrasensitive PCR Detection of Tumor Cells in Myeloma(p. 185)
• 32 Ultrasensitive Quantitative PCR to Detect RNA Viruses(p. 197)
• 33 Quantitative PCR for cAMP RI Alpha mRNA: Use of Site-Directed Mutation and PCR Mimics(p. 205)
• 34 Quantitation of Multiple RNA Species(p. 211)
• Part V Transcriptome Analysis
• 35 Differential Display: A Technical Overview(p. 217)
• 36 AU-Differential Display, Reproducibility of a Differential mRNA Display Targeted to AU Motifs(p. 225)
• 37 PCR Fluorescence Differential Display(p. 237)
• 38 Microarray Analysis Using RNA Arbitrarily Primed PCR(p. 245)
• 39 Oligonucleotide Arrays for Genotyping: Enzymatic Methods for Typing Single Nucleotide Polymorphisms and Short Tandem Repeats(p. 255)
• 40 Serial Analysis of Gene Expression(p. 271)
• Part VI Mutations and Polymorphisms
• 41 Mutation and Polymorphism Detection: A Technical Overview(p. 287)
• 42 Combining Multiplex and Touchdown PCR for Microsatellite Analysis(p. 295)
• 43 Detection of Microsatellite Instability and Loss of Heterozygosity Using DNA Extracted from Formalin-Fixed Paraffin-Embedded Tumor Material by Fluorescence-Based Multiplex Microsatellite PCR(p. 301)
• 44 Reaction of Shadow Band Synthesis During PCR Amplification of Repetitive Sequences from Modern and Ancient DNA(p. 309)
• 45 Degenerate Oligonucleotide-Primed PCR(p. 315)
• 46 Mutation Detection Using RT-PCR-RFLP(p. 319)
• 47 Multiplex Amplification Refractory Mutation System for the Detection of Prothrombotic Polymorphisms(p. 323)
• 48 PCR-SSCP Analysis of Polymorphism: A Simple and Sensitive Method for Detecting Differences Between Short Segments of DNA(p. 327)
• Part VII PCR-Based Sequencing
• 49 Sequencing: A Technical Overview(p. 337)
• 50 Preparation and Direct Automated Cycle Sequencing of PCR Products(p. 341)
• 51 Nonradioactive PCR Sequencing Using Digoxigenin(p. 347)
• 52 Direct Sequencing by Thermal Asymmetric PCR(p. 355)
• 53 Analysis of Nucleotide Sequence Variations by Solid-Phase Minisequencing(p. 361)
• 54 Direct Sequencing with Highly Degenerate and Inosine-Containing Primers(p. 367)
• 55 Determination of Unknown Genomic Sequences Without Cloning(p. 373)
• 56 Cloning PCR Products for Sequencing in M13 Vectors(p. 385)
• 57 DNA Rescue by the Vectorette Method(p. 393)
• 58 Technical Notes for Sequencing Difficult Templates(p. 401)
• Part VIII In Situ PCR and PRINS
• 59 PCR-Based Detection of Nucleic Acids in Chromosomes, Cells, and Tissues: Technical Considerations on PRINS and In Situ PCR, and Comparison with In Situ Hybridization(p. 405)
• 60 Cycling Primed In Situ Amplification(p. 425)
• 61 Direct and Indirect In Situ PCR(p. 433)
• 62 Reverse Transcriptase In Situ PCR: New Methods in Cellular Interrogation(p. 445)
• 63 Primed In Situ Nucleic Acid Labeling Combined with Immunocytochemistry to Simultaneously Localize DNA and Proteins in Cells and Chromosomes(p. 453)
• Part IX Cloning and Mutagenesis
• 64 Cloning and Mutagenesis: A Technical Overview(p. 467)
• 65 Using T4 DNA Polymerase to Generate Clonable PCR Products(p. 469)
• 66 A T-Linker Strategy for Modification and Directional Cloning of PCR Products(p. 475)
• 67 Cloning Gene Family Members Using PCR with Degenerate Oligonucleotide Primers(p. 485)
• 68 cDNA Libraries from a Low Amount of Cells(p. 499)
• 69 Creation of Chimeric Junctions, Deletions, and Insertions by PCR(p. 511)
• 70 Recombination and Site-Directed Mutagenesis Using Recombination PCR(p. 517)
• 71 Megaprimer PCR: Application in Mutagenesis and Gene Fusion(p. 525)
• Index(p. 533)