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Most information on yeasts derives from experiments with the conventional yeasts Saccaromyces cerevisiae and Schizossaccharomyces pombe , the complete nuclear and mitochondrial genome of which has also been sequenced. For all other non-conventional yeasts, investigations are in progress and the rapid development of molecular techniques has allowed an insight also into a variety of non-conventional yeasts.
In this bench manual, over 70 practical protocols using 15 different non-conventional yeast species and in addition several protocols of general use are described in detail. All of these experiments on the genetics, biochemistry and biotechnology of yeasts have been contributed by renowned laboratories and have been reproduced many times. The reliable protocols are thus ideally suited also for undergraduate and graduate practical courses.

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• Arxula adeninivorans
• Chapter 1 Temperature-Dependent Dimorphism of the Non-Conventional Yeast Arxula adeninivorans(p. 3)
• Chapter 2 Expression of Heterologous Genes in Arxula adeninivorans Budding Cells and Mycelia(p. 7)
• Ashbya gossypii
• Chapter 3 Effect of Precursor Feeding on Riboflavin Production of Ashbya gossypii and Influence of Threonine Aldolase Overexpression(p. 15)
• Candida maltosa
• Chapter 4 Characterization of the 26S-rRNA Gene to Classify an Industrial Strain to be Candida maltosa(p. 23)
• Chapter 5 Isolation of a GCN4 Gene Analog and Determination of Its Involvement in Induction of Cycloheximide-Resistant Ribosomal Protein LA1-Q in Candida maltosa(p. 29)
• Chapter 6 Dehalogenation During Biodegradation of Chlorophenols by Candida maltosa(p. 35)
• Chapter 7 Production of Aromatic Acids During the Oxidation of Phenylalkanes by Candida maltosa(p. 39)
• Debaryomyces hansenii
• Chapter 8 Growth of Debaryomyces hansenii in Seawater Culture Medium(p. 47)
• Chapter 9 Glycerol and Glucose Dissimilation by Debaryomyces hansenii: Substrate Influence on Growth Rate and Biomass Yield of Yeast(p. 51)
• Chapter 10 Induction of GDP from Halotolerant Yeast Debaryomyces hansenii by Salt Stress(p. 59)
• Chapter 11 Salt Tolerance in Debaryomyces hansenii. Effect of Sodium on Growth at High Temperature and at Extreme pH(p. 65)
• Chapter 12 Preparation of Total DNA from Debaryomyces hansenii(p. 69)
• Hansenula polymorpha
• Chapter 13 Life Cycle of Hansenula polymorpha(p. 77)
• Chapter 14 Obtention of Auxotrophic Mutants in Hansenula polymorpha(p. 83)
• Chapter 15 Transformation by Electroporation of the Yeast Hansenula polymorpha(p. 89)
• Chapter 16 Expression of Hansenula polymorpha Nitrate Reductase Under the Control of the MOX1 Promoter(p. 97)
• Chapter 17 Promoter Assessment in H. polymorpha Using a lacZ Reporter Gene(p. 101)
• Chapter 18 Immuno-Gold Labeling of Invertase in Hansenula polymorpha(p. 117)
• Hortaea werneckii
• Chapter 19 Determination of Intra- and Extracellular Concentration of Glycerol in the Halophilic Black Yeast Hortaea werneckii Grown at Different Environmental Salinities(p. 127)
• Chapter 20 Regulation of HMGCoA Reductase Activity by Salt Stress in Hortaea werneckii(p. 131)
• Chapter 21 Isolation and Identification of Lipids from the Halophilic Black Yeast Hortaea werneckii(p. 135)
• Chapter 22 Inhibition of DHN-Melanin Biosynthesis by Tricyclazole in Hortaea werneckii(p. 143)
• Kluyveromyces lactis
• Chapter 23 Mating, Sporulation, and Tetrad Analysis in Kluyveromyces lactis(p. 151)
• Chapter 24 Isolation of Mitochondrial Mutants from the Petite-Negative Yeast Kluyveromyces lactis(p. 155)
• Chapter 25 Gene Disruption in the Yeast Kluyveromyces lactis(p. 161)
• Chapter 26 Transformation of Kluyveromyces lactis(p. 169)
• Chapter 27 Removal of Dominant Markers from the Kluyveromyces lactis Genome Using the Cre/loxP System(p. 175)
• Chapter 28 Phenotypic Analysis of the Kluyveromyces lactis Killer Phenomenon(p. 179)
• Chapter 29 Genetic Manipulation of the Kluyveromyces lactis Killer Plasmids k1 and k2(p. 185)
• Chapter 30 Chitin-Binding Capacity of the Zymocin Complex from Kluyveromyces lactis(p. 191)
• Chapter 31 Heterologous Protein Production in High Copy Number Vector Systems(p. 185)
• Chapter 32 Alcohol Dehydrogenase (ADH) Isozymes in K. lactis: Detection by Activity(p. 201)
• Chapter 33 Kluyveromyces lactis Genome Mapping and Sequencing(p. 209)
• Pichia stipitis
• Chapter 34 Mating and Segregation in Pichia stipitis(p. 215)
• Chapter 35 ADH1 Disruption in P. stipitis by Targeted Restriction Enzyme Mediated Integration(p. 221)
• Chapter 36 Examination of Xylose Fermentation in P. stipitis by Means of the Sensomat System(p. 229)
• Chapter 37 Regulation of Fermentation and Respiration(p. 236)
• Chapter 38 Investigation of Transcriptional Regulation of the Fermentative ADH in Pichia stipitis Using an EGFP Reporter Gene(p. 241)
• Chapter 39 Cyanide-Insensitive Respiration(p. 245)
• Chapter 40 Freeze Transformation, Plasmid Reisolation and Stability in Pichia stipitis(p. 253)
• Schwanniomyces occidentalis
• Chapter 41 Objective Analysis of Medium-Induced Fragility of Schwanniomyces occidentalis(p. 263)
• Chapter 42 Subjective Analysis of Medium-Induced Fragility of Schwanniomyces occidentalis(p. 267)
• Chapter 43 Analysis of Structural Discontinuities of the Wall of Schwanniomyces occidentalis(p. 271)
• Chapter 44 Induction of Amylases in Schwanniomyces occidentalis(p. 275)
• Chapter 45 Ethanol Production from Starch by Schwanniomyces occidentalis(p. 279)
• Trichosporon domesticum
• Chapter 46 Characterization of Soluble Cytochrome P450 from Trichosporon domesticum(p. 285)
• Trichosporon montevideense
• Chapter 47 Oxidation of the Fungicide Biphenyl by Yeasts of the Genus Trichosporon(p. 293)
• Xanthophyllomyces dendroorhous
• Chapter 48 Genetic Complementation Analysis by Protoplast Fusion of Xanthophyllomyces dendrorhous(p. 299)
• Chapter 49 Lethal Effect of UV Light and Photoreactivation in Xanthophyllomyces dendrorhous(p. 305)
• Chapter 50 Fermentation and Carotenoid Analysis of the Yeast Xanthophyllomyces dendrorhous (Phaffia rhodozyma)(p. 309)
• Chapter 51 Metabolic Engineering of the Carotenoid Biosynthetic Pathway in Xanthophyllomyces dendrorhous (Phaffia rhodozyma)(p. 315)
• Chapter 52 Astaxanthin Biosynthesis in Xanthophyllomyces dendrorhous (Phaffia rhodozyma): Effect of Inhibitors and Low Temperature(p. 323)
• Chapter 53 Purification and Characterization of Extrachromosomal Genetic Elements of Double-Stranded RNA (dsRNA) of Xanthophyllomyces dendrorhous(p. 329)
• Yarrowia lipolytica
• Chapter 54 Mating, Sporulation and Random Spore Selection by Nystatin in Yarrowia lipolytica(p. 335)
• Chapter 55 Induction of the Yeast-Hypha Transition in Yarrowia lipolytica(p. 339)
• Chapter 56 Tagging of Genes by Insertional Mutagenesis in the Yeast Yarrowia lipolytica(p. 343)
• Chapter 57 Detection and Activation of the Ylt1 Retrotransposon in the Yeast Yarrowia lipolytica(p. 357)
• Chapter 58 Flow Cytometry of Yarrowia lipolytica(p. 365)
• Chapter 59 A Copper-Regulated Yarrowia lipolytica Vector for the Expression of Heterologous Proteins(p. 369)
• Chapter 60 Lipase Assay in Yarrowia lipolytica(p. 373)
• Chapter 61 Expression of Mutant Gpr1p in Yarrowia lipolytica and Its Effect on Growth on Different Media(p. 379)
• Chapter 62 Detection of Biosynthesis and Degradation of Peroxisomes by Different Carbon Sources with a Combined Assay Using a [beta]-Galactosidase-Gfp-SKL Chimaeric Protein for Labeling of Peroxisomes and the Peroxisomal Isocitrate Lyase as Control|cI. Parshyna(p. 385)
• Chapter 63 Induction of Citric Acid/Isocitric Acid and [alpha]-Ketoglutaric Acid Production in the Yeast Yarrowia lipolytica(p. 393)
• Chapter 64 Regulation of Production of Yarrowia lipolytica Extracellular Ribonuclease and Alkaline Extracellular Protease(p. 401)
• Zygosaccharomyces bailii
• Chapter 65 Zygosaccharomyces bailii: A Yeast with a Peculiar Pattern for the Regulation of Acetic Acid Metabolism in the Presence of Glucose(p. 409)
• Chapter 66 Transfection of Yeast with Toxin-Encoding dsRNA Viruses of Zygosaccharomyces bailii and Toxin Purification by Receptor-Mediated Affinity Chromatography(p. 417)
• Zygosaccharomyces rouxii
• Chapter 67 Heat-Shock-Induced Activation of Trehalase in the Yeast Zygosaccharomyces rouxii(p. 429)
• Miscellaneous Non-Conventional Yeasts
• Chapter 68 Linear and Circular Plasmids of Yeasts(p. 437)
• Chapter 69 Rapid Method to Extract DNA from Capsulated Yeast Species(p. 445)
• Chapter 70 Testing Various Yeasts for Ethanol Production from Xylose(p. 451)
• Chapter 71 Use of a Differential Culture Medium for the Enumeration of Zygosaccharomyces bailii, Saccharomyces cerevisiae and Pichia membranifaciens in Wine(p. 457)
• Chapter 72 Characterization of Ubiquinone Type (CoQ7-CoQ10) of Non-Conventional Yeasts(p. 463)
• Chapter 73 Non-Radioactive Dot Blot DNA Reassociation for Unequivocal Yeast Identification(p. 469)
• Chapter 74 Taxonomy Rules and Classification Hints for Non-Taxonomists Working with Unknown or Ill-Identified Yeast Cultures(p. 475)
• Subject Index(p. 491)