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Calculations for Molecular Biology and Biotechnology: A Guide to Mathematics in the Laboratory, Second Edition , provides an introduction to the myriad of laboratory calculations used in molecular biology and biotechnology. The book begins by discussing the use of scientific notation and metric prefixes, which require the use of exponents and an understanding of significant digits. It explains the mathematics involved in making solutions; the characteristics of cell growth; the multiplicity of infection; and the quantification of nucleic acids. It includes chapters that deal with the mathematics involved in the use of radioisotopes in nucleic acid research; the synthesis of oligonucleotides; the polymerase chain reaction (PCR) method; and the development of recombinant DNA technology. Protein quantification and the assessment of protein activity are also discussed, along with the centrifugation method and applications of PCR in forensics and paternity testing.

Tabla de contenidos provista por Syndetics

• Chapter 1 Scientific Notation and Metric Prefixes
• Chapter 2 Solutions, Mixtures, and Media
• Chapter 3 Cell Growth
• Chapter 4 Working with Bacteriophage
• Chapter 5 Quantitation of Nucleic Acids
• Chapter 6 Labeling Nucleic Acids with Radioisotopes
• Chapter 7 Oligonucleotide Synthesis
• Chapter 8 The Polymerase Chain Reaction
• Chapter 9 Real Time PCR
• Chapter 10 Recombinant DNA
• Chapter 11 Protein
• Chapter 12 Centrifugation
• Chapter 13 Forensic Science

Notas de autor provistas por Syndetics

Frank Stephenson received his doctorate in molecular biology from UC Berkeley and has published several books in the field including 'DNA: How the Biotech Revolution is Changing the Way We Fight Disease' and 'A Hands-On Introduction to Forensic Science: Cracking the Case'. He is currently an instructor in the Technical Training Department with ThermoFisher Scientific, the world's leading manufacturer of instrumentation and reagents for the biotechnology industry.