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Transgenic plants methods and protocols edited by Leandro Peña.
Series Methods in molecular biology (Clifton, N.J.) ; v. 286.Detalles de publicación: Totowa, N.J. Humana Press c2005.Descripción: xiv, 437 p. ill. (some col.) 24 cmISBN:- 1588292630 (alk. paper)
- 631.5233 T7724 22
- SB123.57 .T728 2005
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| Imagen de cubierta | Tipo de ítem | Biblioteca actual | Biblioteca de origen | Colección | Ubicación en estantería | Signatura topográfica | Materiales especificados | Info Vol | URL | Copia número | Estado | Notas | Fecha de vencimiento | Código de barras | Reserva de ítems | Prioridad de la cola de reserva de ejemplar | Reservas para cursos | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Libro | Biblioteca Encarnación Valdés Colección General bev | 631.5233 T7724 (Navegar estantería(Abre debajo)) | Disponible | 80000002440447 |
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Descripciones mejoradas de Syndetics:
Transgenic Plants: Methods and Protocols provides a wide range of frequently used, broadly applicable and easily reproducible techniques involved in the generation of transgenic plants. Its step-by-step approach covers methods for genetically transforming plants, cells, and tissues and for recovering whole transgenic plants from them. Also covered are the use of selectable and reporter markers, posititve selection, marker elimination after recovery of transgenic plants, and analysis of transgene integration, expression, and localization in the plant genome.
Includes bibliographical references.
Tabla de contenidos provista por Syndetics
- Preface(p. v)
- Contributors(p. xi)
- Part I. Introduction
- 1. Transgenic Plants: An Historical Perspective(p. 3)
- Part II. Transformation
- 2. Plant Transformation: Agrobacterium-Mediated Gene Transfer(p. 35)
- 3. Production of Hairy Root Cultures and Transgenic Plants by Agrobacterium rhizogenes-Mediated Transformation(p. 47)
- 4. Stable Transformation of Plant Cells by Particle Bombardment/Biolistics(p. 61)
- 5. Electroporation: Introduction and Expression of Transgenes in Plant Protoplasts(p. 79)
- 6. Floral Dip: Agrobacterium-Mediated Germ Line Transformation(p. 91)
- 7. Production of Transgenic Crops by the Floral-Dip Method(p. 103)
- 8. Chloroplast Genetic Engineering to Improve Agronomic Traits(p. 111)
- Part III. Regeneration
- 9. Organogenesis From Transformed Tomato Explants(p. 141)
- 10. Genetic Transformation of Conifers Utilizing Somatic Embryogenesis(p. 151)
- 11. Regeneration of Transgenic Cassava From Transformed Embryogenic Tissues(p. 165)
- 12. Genetic Transformation of Mature Citrus Plants(p. 177)
- Part IV. Selection
- 13. Selectable Markers: Antibiotic and Herbicide Resistance(p. 191)
- 14. Histochemical and Fluorometric Assays for uidA (GUS) Gene Detection(p. 203)
- 15. Green Fluorescent Protein Quantification in Whole Plants(p. 215)
- 16. Positive Selection(p. 227)
- 17. Elimination of Marker Genes From Transgenic Plants Using MAT Vector Systems(p. 237)
- 18. Simple and Efficient Removal of Marker Genes From Plastids by Homologous Recombination(p. 255)
- Part V. Transgene Integration, Expression, and Localization
- 19. The Study of Transgene Copy Number and Organization(p. 273)
- 20. Analysis of Gene Expression in Transgenic Plants(p. 291)
- 21. Transgene Integration: Use of Matrix Attachment Regions(p. 313)
- 22. Fluorescence In Situ Hybridization to Localize Transgenes in Plant Chromosomes(p. 327)
- 23. Amplification of Genomic Sequences Flanking T-DNA Insertions by Thermal Asymmetric Interlaced Polymerase Chain Reaction(p. 341)
- Part VI. Risk Assessment
- 24. Agrobacterium Persistence in Plant Tissues After Transformation(p. 351)
- 25. Transgene Dispersal Through Pollen(p. 365)
- Part VII. Transgenic Crops
- 26. Transgenic Crops: The Current and Next Generations(p. 377)
- 27. Comparative Development and Impact of Transgenic Papayas in Hawaii, Jamaica, and Venezuela(p. 399)
- Index(p. 431)