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High performance liquid chromatography, (HPLC), is the most powerful of all chromatograhpic techniques; it is capable of achieving separations and analyses difficult or impossible to perform by other methods. Its sophistication requires a solid theoretical background as well as extensive experience if it is to be used effectively. This volume explains the theory and practice of HPLC, with detailed instruction on its uses for separation and quantitative analysis, practical information on the equipment needed, and the various parameters that control HPLC separations. Readers will be able to process an analysis in a logical manner and to determine what to alter, and how, in order to obtain results. The format of this text encourages independent, self-paced study, and suggested experiments are included to encourage readers to apply their knowledge. A bibliography is also included to keep readers up to date on this rapidly expanding field.