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Spiral bound standardized lab guide for teachers, students, researchers, extension personnel, and others who identify plant bacterial diseases. Includes recently developed tests. Annotation(c) 2003 Book News, Inc., Portland, OR (booknews.com)

Tabla de contenidos provista por Syndetics

• Contributors(p. III)
• Preface to the Third Edition(p. VI)
• I. Initial Identification of Common Genera
• A. Introduction(p. 1)
• B. Isolation Techniques Using Differential and Semiselective Media(p. 3)
• C. Differentiation of Commonly Isolated Genera(p. 6)
• D. Diagnostic Media and Tests(p. 7)
• E. Literature Cited(p. 14)
• F. Chemicals List(p. 15)
• II. Gram-Negative Bacteria
• A. Agrobacterium
• 1. Introduction(p. 17)
• 2. Isolation Techniques Using Semiselective Media(p. 18)
• 3. Differentiation of Commonly Isolated Species(p. 22)
• 4. Diagnostic Media and Tests(p. 24)
• 5. Pathogenicity Tests(p. 26)
• 6. Molecular, Serological, and Commercial Automated Techniques(p. 28)
• 7. Culture Preservation(p. 30)
• 8. Literature Cited(p. 30)
• 9. Chemicals List(p. 34)
• B. Erwinia and Pantoea
• 1. Introduction(p. 36)
• 2. Literature Cited(p. 37)
• B-1. Erwinia amylovora Group
• 1. Introduction(p. 40)
• 2. Isolation Techniques Using Differential and Semiselective Media(p. 40)
• 3. Differentiation of Commonly Isolated Species(p. 44)
• 4. Diagnostic Media and Tests(p. 44)
• 5. Pathogenicity Tests(p. 48)
• 6. Molecular, Serological, and Commercial Automated Techniques(p. 49)
• 7. Culture Preservation(p. 51)
• 8. Literature Cited(p. 51)
• 9. Chemicals List(p. 54)
• B-2. Erwinia Soft Rot Group
• 1. Introduction(p. 56)
• 2. Isolation Techniques Using Semiselective Media(p. 56)
• 3. Differentiation of Commonly Isolated Species and Subspecies(p. 58)
• 4. Diagnostic Media and Tests(p. 59)
• 5. Pathogenicity Tests(p. 62)
• 6. Molecular, Serological, and Commercial Automated Techniques(p. 63)
• 7. Culture Preservation(p. 65)
• 8. Literature Cited(p. 66)
• 9. Chemicals List(p. 71)
• B-3. Pantoea
• 1. Introduction(p. 73)
• 2. Isolation Techniques Using Differential and Semiselective Media(p. 73)
• 3. Differentiation of Commonly Isolated Species(p. 76)
• 4. Diagnostic Media and Tests(p. 76)
• 5. Pathogenicity Tests(p. 78)
• 6. Molecular, Serological, and Commercial Automated Techniques(p. 79)
• 7. Culture Preservation(p. 80)
• 8. Literature Cited(p. 81)
• 9. Chemicals List(p. 83)
• C. Pseudomonas
• 1. Introduction(p. 84)
• 2. Isolation Techniques Using Differential and Semiselective Media(p. 85)
• 3. Differentiation of Commonly Isolated Species and Pathovars(p. 90)
• 4. Diagnostic Media and Tests(p. 93)
• 5. Pathogenicity Tests(p. 102)
• 6. Molecular, Serological, and Commercial Automated Techniques(p. 104)
• 7. Culture Preservation(p. 108)
• 8. Literature Cited(p. 111)
• 9. Chemicals List(p. 117)
• D. Acidovorax and Xylophilus
• 1. Introduction(p. 121)
• 2. Isolation Techniques Using Differential and Semiselective Media(p. 122)
• 3. Differentiation of Commonly Isolated Species and Subspecies(p. 126)
• 4. Diagnostic Media and Tests(p. 128)
• 5. Pathogenicity Tests(p. 132)
• 6. Molecular, Serological, and Commercial Automated Techniques(p. 133)
• 7. Culture Preservation(p. 134)
• 8. Literature Cited(p. 135)
• 9. Chemicals List(p. 137)
• E. Burkholderia
• 1. Introduction(p. 139)
• 2. Isolation Techniques Using Differential and Semiselective Media(p. 140)
• 3. Differentiation of Commonly Isolated Species(p. 142)
• 4. Diagnostic Media and Tests(p. 142)
• 5. Pathogenicity Tests(p. 145)
• 6. Molecular, Serological, and Commercial Automated Techniques(p. 145)
• 7. Culture Preservation(p. 147)
• 8. Literature Cited(p. 147)
• 9. Chemicals List(p. 150)
• F. Ralstonia
• 1. Introduction(p. 151)
• 2. Isolation Techniques Using Differential and Semiselective Media(p. 153)
• 3. Differentiation of Commonly Isolated Species and Subspecies(p. 155)
• 4. Diagnostic Media and Tests(p. 157)
• 5. Pathogenicity Tests(p. 162)
• 6. Molecular, Serological, and Commercial Automated Techniques(p. 163)
• 7. Culture Preservation(p. 166)
• 8. Literature Cited(p. 167)
• 9. Chemicals List(p. 173)
• G. Xanthomonas
• 1. Introduction(p. 175)
• 2. Identification of Xanthomonadin Pigment(p. 177)
• 3. Isolation Techniques Using Differential and Semiselective Media(p. 178)
• 4. Differentiation of Commonly Isolated Species and Pathovars(p. 188)
• 5. Diagnostic Media and Tests(p. 189)
• 6. Pathogenicity Tests(p. 190)
• 7. Molecular, Serological, and Commercial Automated Techniques(p. 191)
• 8. Culture Preservation(p. 193)
• 9. Literature Cited(p. 194)
• 10. Chemicals List(p. 199)
• H. Xylella fastidiosa
• 1. Introduction(p. 201)
• 2. Isolation Techniques Using Differential Media(p. 201)
• 3. Differentiation of Commonly Isolated Pathotypes(p. 203)
• 4. Diagnostic Media and Tests(p. 204)
• 5. Pathogenicity Tests(p. 207)
• 6. Molecular, Serological, and Commercial Automated Techniques(p. 208)
• 7. Culture Preservation(p. 210)
• 8. Literature Cited(p. 210)
• 9. Chemicals List(p. 213)
• I. Rhizomonas suberifaciens
• 1. Introduction(p. 214)
• 2. Isolation Technique Using Semiselective Medium(p. 214)
• 3. Differentiation of Commonly Isolated Species(p. 215)
• 4. Diagnostic Media and Tests(p. 215)
• 5. Pathogenicity Tests(p. 215)
• 6. Culture Preservation(p. 216)
• 7. Literature Cited(p. 216)
• 8. Chemicals List(p. 217)
• III. Gram-Positive Bacteria
• A. Coryneform Plant Pathogens
• 1. Introduction(p. 218)
• 2. Isolation Techniques Using Semiselective Media(p. 220)
• 3. Differentiation of Commonly Isolated Species and Subspecies(p. 223)
• 4. Diagnostic Media and Tests(p. 225)
• 5. Pathogenicity Tests(p. 226)
• 6. Molecular, Serological, and Commercial Automated Techniques(p. 227)
• 7. Culture Preservation(p. 229)
• 8. Literature Cited(p. 230)
• 9. Chemicals List(p. 234)
• B. Streptomyces
• 1. Introduction(p. 236)
• 2. Isolation Techniques Using Differential and Semiselective Media(p. 236)
• 3. Differentiation of Commonly Isolated Species(p. 238)
• 4. Diagnostic Media and Tests(p. 240)
• 5. Pathogenicity Tests(p. 243)
• 6. Molecular Techniques(p. 245)
• 7. Culture Preservation(p. 245)
• 8. Literature Cited(p. 246)
• 9. Chemicals List(p. 249)
• C. Bacillus
• 1. Introduction(p. 250)
• 2. Isolation Techniques Using Differential Media(p. 251)
• 3. Differentiation of Commonly Isolated Species(p. 252)
• 4. Diagnostic Media and Tests(p. 253)
• 5. Pathogenicity Tests(p. 256)
• 6. Commercial Automated Techniques(p. 256)
• 7. Culture Preservation(p. 256)
• 8. Literature Cited(p. 257)
• 9. Chemicals List(p. 259)
• D. Clostridium
• 1. Introduction(p. 261)
• 2. Isolation Techniques Using Differential and Semiselective Media(p. 262)
• 3. Differentiation of Commonly Isolated Species(p. 265)
• 4. Diagnostic Media and Tests(p. 268)
• 5. Inoculation Tests for Maceration of Tissue(p. 270)
• 6. Literature Cited(p. 270)
• 7. Chemicals List(p. 274)
• IV. Fastidious Phloem-Limited Bacteria
• A. Introduction(p. 275)
• B. Isolation Techniques Using Selective Agar Media(p. 276)
• C. Differentiation of Commonly Isolated Species(p. 276)
• D. Diagnostic Media and Tests(p. 277)
• E. Pathogenicity Tests(p. 277)
• F. Molecular, Serological, and Commercial Automated Techniques(p. 278)
• G. Culture Preservation(p. 278)
• H. Literature Cited(p. 279)
• V. Cell-Wall Free Bacteria
• A. Introduction(p. 283)
• B. Isolation Techniques(p. 285)
• C. Differentiation of Species or Groups(p. 293)
• D. Diagnostic Tests(p. 296)
• E. Pathogenicity Tests(p. 299)
• F. Molecular, Serological, and Commercial Automated Techniques(p. 303)
• G. Literature Cited(p. 311)
• H. Chemicals List(p. 320)
• VI. Appendix
• A. Molecular Techniques
• 1. Introduction(p. 321)
• 2. DNA Extraction(p. 322)
• 3. PCR-Based Methods(p. 323)
• 4. PER-PCR Genomic Fingerprint Analysis to Characterize and Identify Phytobacteria(p. 325)
• 5. Literature Cited(p. 328)
• 6. Diagnostic Companies(p. 333)
• A-1. DNA Isolation Procedure
• 1. Introduction(p. 334)
• B. Serological Techniques
• 1. Immunodiagnostic Methods(p. 338)
• 2. Literature Cited(p. 339)
• 3. Diagnostic Companies(p. 341)
• C. Automated Techniques
• 1. Physiological and Biochemical Methods(p. 343)
• 2. Molecular Methods(p. 347)
• 3. Commercial Laboratories(p. 350)
• 4. Literature Cited(p. 350)
• 5. Diagnostic Companies(p. 356)
• VII. Index(p. 359)
• Color plates(p. 260)