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Laboratory Exercises in Microbiology, 7/e has been prepared to accompany Prescott, Harley and Klein's Microbiology, 7e, written by new authors Joanne Willey, Linda Sherwood, and Christopher Woolverton. Like the text, the laboratory manual provides a balanced introduction to laboratory techniques and principles that are important in each area of microbiology..

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• 1 Microscopic Techniques
• 1 Bright-Field Light Microscope and Microscopic Measurement of Organisms
• 2 The Hanging Drop Slide and Bacterial Motility
• 3 Dark-Field Light Microscope
• 4 Phase-Contrast Light Microscope
• 5 Flourescence Microscope
• 2 Bacterial Morphology and Staining
• 6 Negative Staining
• 7 Smear Preparation and Simple Staining
• 8 Gram Stain
• 9 Acid-Fast Staining (Ziehl-Neelsen and Kinyoun) Procedures
• 10 Endospore Staining (Schaeffer-Fulton or Wirtz-Conklin)
• 11 Capsule Staining
• 12 Flagella Staining: West and Difco's SpotTest Methods
• 3 Basic Laboratory and Culture Techniques
• 13 Microbiological Culture Media Preparation and Sterilization
• 14 Culture Transfer Instruments, Techniques, and Isolation and Maintenance of Pure Cultures
• 15 Spread-Plate Technique
• 16 The Streak-Plate Technique and Different Media
• 17 Pour-Plate Technique
• 18 Cultivation of Anaerobic Bacteria
• 19 Determination of Bacterial Numbers
• 4 Biochemical Activities of Bacteria
• 20 Carbohydrates I: Fermentation and B-Galactosidase Activity
• 21 Carbohydrates II: Triple Sugar Iron Agar Tests
• 22 Carbohydrates III: Starch Hydrolysis
• 23 Lipids: Lipid Hydrolysis
• 24 Proteins, Amino Acids, and Enzymes I: Hydrogen Sulfide Production and Motility
• 25 Proteins, Amino Acids, and Enzymes II: The IMViC Tests
• 26 Proteins, Amino Acids, and Enzymes III: Casein Hydrolysis
• 27 Proteins, Amino Acids, and Enzymes IV: Gelatin Hydrolysis
• 28 Proteins, Amino Acids, and Enzymes V: Catalase Activity
• 29 Proteins, Amino Acids, and Enzymes VI: Coagulase and DNase Activity
• 30 Proteins, Amino Acids, and Enzymes VII: Oxidase Test
• 31 Proteins, Amino Acids, and Enzymes VIII: Urease Activity
• 32 Proteins, Amino Acids, and Enzymes IX: Lysine and Ornithine Decarboxylase Test
• 33 Proteins, Amino Acids, and Enzymes X: Phenylalanine Deamination
• 34 Proteins, Amino Acids, and Enzymes XI: Nitrate Reduction
• 5 Rapid Multitest Systems
• 35 The API 20E System
• 36 The Enterotube II System
• 6 Unknown Identification
• 37 Using the First Edition of Bergey's Manual of Systematic Bacteriology to Identify Bacteria
• 38 General Unknown
• 7 Environmental Factors Affecting Growth of Microorganisms
• 39 Temperature
• 40 pH
• 41 Osmotic Pressure
• 42 The Effects of Chemical Agents on Bacteria I: Disinfectants
• 43 The Effects of Chemical Agents on Bacteria II: Antimicrobial Agents (Kirby-Bauer Method)
• 44 Hand Washing, Environmental Sampling, and Microbiological Monitoring
• 45 Determination of a Bacterial Growth Curve: Classical and Two-Hour Methods
• 8 Environmental and Food Microbiology
• 46 Standard Coliform Most Probably Number (MPN)Test and Presence-Absence Coliform Test
• 47 Membrane Filter Technique for Coliform and Fecal Streptococci; KONFIRM Test for Fecal Coliforms
• 48 Isolation of Escherichia coli Bacteriophages from Sewage and Determining Bacteriophage Titers
• 49 Enumeration of Soil Microorganisms
• 50 Bacterial Count of a Food Product
• 51 Examination of Milk for Bacteria
• 9 Medical Microbiology
• 52 Agglutination Reactions: Blood Groups
• 53 Isolation of Normal Microbiota from the Human Body
• 54 Staphylococci
• 55 Pneumococci
• 56 Streptococci
• 57 Neisseriae
• 58 Aerobic and Anaerobic Endospore-Forming Bacteria
• 10 Survey of Selected Eucaryotic Microorganisms
• 59 Fungi I: Yeasts
• 60 Fungi II: Phycomycetes, Ascomycetes, and Basidiomycetes
• 11 Microbial Genetics and Genomics
• 61 Bacterial Mutation
• 62 Bacterial Transformation
• 63 Bacterial Conjugation: The Transfer of Antibiotic-Resistant Plasmids
• 64 Isolation of Genomic DNA from Saccharomyces cerevisiae
• 65 Isolation and Purification of Genomic DNA from Escherichia coli
• 66 Identifying Archaea and Bacteria Using the Internet and Computer-Assisted Gene Analysis
• Appendix A Dilutions with Sample Problems
• Appendix B Metric and English Measurement Equivalents
• Appendix C Transmission-Absorbance Ta