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Written by exceptionally experienced experts from one of the world's major biotech regions, the Heidelberg area, the authors come from academia, such well-known research centers as the German Cancer Research Center, and biotech companies.
A very detailed introduction to the fundamentals in molecular and cell biology is followed by an overview of standard techniques applied in molecular biotechnology -- including chromatography and electrophoresis, cloning techniques, gene expression systems, immunological methods, labeling of proteins and in situ-techniques, microscopy and laser systems. The third part then focuses on the key topics of molecular biotechnology, ranging from functional genomics, proteomics and bioinformatics to drug targeting, recombinant antibodies, structural biology, gene therapy and knock mice. The whole is rounded off by a section on biotechnology in industry dealing with patenting issues, company foundation and market opportunities.
On over 800 pages this book provides students and professionals in life sciences, pharmacy and biochemistry with all they need to know about molecular biotechnology.

Tabla de contenidos provista por Syndetics

• Preface
• List of Contributors
• Abbreviations
• Colour Plates
• Part I: Fundamentals of Cellular and Molecular Biology
• 1 The Cell as the Basic Unit of Life
• 2 Structure and Function of Cellular Macromolecules
• 2.1 Introduction
• 2.2 Structure and Function of Sugars
• 2.3 Structure of Membrane Lipids
• 2.4 Structure and Function of Proteins
• 2.5 Structure of Nucleotides and Nucleic Acids (DNA and RNA)
• 3 Structure and Function of a Cell
• 3.1 The Structure of a Eukaryotic Cell
• 3.2 The Structure of Bacteria
• 3.3 The Structure of Viruses
• 3.4 The Differentiation of Cells
• 4 Biosynthesis and Function of Macromolecules (DNA, RNA, and Proteins)
• 4.1 Genomes, Chromosomes, and Replication
• 4.2 Transcription: From Gene to Protein
• 4.3 Protein Biosynthesis (Translation)
• 5 Distributing Proteins in the Cell (Protein Sorting)
• 5.1 Import and Export of Proteins via the Nuclear Pore
• 5.2 Import of Proteins in Mitochondria and Chloroplasts
• 5.3 Protein Transport in the Endoplasmic Reticulum
• 5.4 Vesicle Transport from the ER via the Golgi Apparatus to the Cytoplasmic Membrane
• 6 Diversity of Organisms
• 6.1 Prokaryotes
• 6.2 Eukaryotes
• Further Reading for Chapters 1-6
• Part II Standard Methods in Molecular Biotechnology
• 7 Isolation and Purification of Proteins
• 7.1 Introduction
• 7.2 Producing a Protein Extract
• 7.3 Gel Electrophoretic Separation Methods
• 7.4 Methods of Protein Precipitation
• 7.5 Column Chromatography Methods
• 7.6 Examples
• Further Reading
• 8 Peptide and Protein Analysis with Electrospray Tandem Mass Spectrometry
• 8.1 Introduction
• 8.2 Principles of Mass Spectrometry
• 8.3 Mass Precision, Resolution, and Isotope Distribution
• 8.4 Principles of Electrospray Ionization
• 8.5 Tandem Mass Spectrometers
• 8.6 Peptide Sequencing with MS/MS
• 8.7 Identifying Proteins with MS/MS-Data and Protein Databases
• 8.8 Determining Protein Molecular Mass
• 8.9 Analysis of Covalent Protein Modification
• Further Reading
• 9 Isolation of DNA and RNA
• 9.1 Introduction
• 9.2 DNA Isolation
• 9.3 RNA Isolation
• l9.3.1
• References
• 10 Chromatography and Electrophoresis of Nucleic Acids
• 10.1 Introduction
• 10.2 Chromatographic Separation of Nucleic Acids
• 10.3 Electrophoresis
• Further Reading
• 11 Hybridization of Nucleic Acids
• 11.1 The Significance of Base Pairing
• 11.2 Experimental Hybridization, Kinetic, and Thermodynamic Control
• 11.3 Analytical Techniques
• Further Reading
• 12 The Use of Enzymes in the Modification of Nucleic Acids
• 12.1 Restriction Enzymes (Restriction Endonucleases)
• 12.2 Ligases
• 12.3 Methylases
• 12.4 DNA Polymerases
• 12.5 Nucleases
• 12.6 T4 Polynucleotide Kinase
• 12.7 Calf Intestinal Phosphatase
• Further Reading
• 13 Polymerase Chain Reaction (PCR)
• 13.1 Introduction
• 13.2 Techniques
• 13.3 Areas of Application
• Further Reading
• 14 DNA Sequencing
• 14.1 Introduction
• 14.2 DNA Sequencing Methods
• 14.3 Strategies for Sequencing the Human Genome
• 14.4 The Practica

Notas de autor provistas por Syndetics

Professor Michael Wink studied Biology in Bonn and Braunschweig, Germany. After post-doctoral work in Cologne and Munich he took up a professorship at the University of Mainz, Germany. Since 1989 Michael Wink has been Professor of Pharmaceutical Biology at the University of Heideberg and was appointed Director of the Institute of Pharmacy and Molecular Biotechnology in 2002. His scientific interests focus on bioactive natural products, pharmacology & toxicology of natural products, and molecular evolution. Professor Wink is author of numerous scientific papers and pharmaceutical biology books.