Descripciones mejoradas de Syndetics:

This masterful third edition of Freshney's Culture of Animal Cells updates and considerably expands the scope of its predecessor and still enables both the novice and the experiences researcher to apply the basic and more sophisticated techniques of tissue culture.

New Topics covered include:

the use of molecular techniques in cell culture, such as DNA fingerprinting, fluorescence in situ hybridization, and chromosome painting cell interactions in cell culture new methods for separating cells new or refined methods for accessing cytotoxicity, viability, and mutagenicity experimental details for culture of specialized cells types not covered in previous editions new or refined techniques for visualizing clues, including time-lapse photography and confocal microscopy

The revised and expanded third edition offers the following features:

over 350 new reference to the primary literature an international list of cell banks an international listing of reagants and commercial supplies a subject index a glossary

Also available: 0471169021 Culture of Animal Cells: A Multimedia Guide CD-ROM $150 est.

From the reviews:

"I strongly recommend this volume for any laboratory wishing to culture mammalian cells" - Biotechnology

"It is not very often that it is possible to say of a book, 'I don't know how I managed without it previously.' Here is such a book" - Cell Biology International Reports

Tabla de contenidos provista por Syndetics

• Figures(p. xvii)
• Color Plates(p. xxi)
• Preface(p. xxiii)
• Abbreviations(p. xxv)
• 1 Introduction(p. 1)
• Background(p. 1)
• Advantages of Tissue Culture(p. 4)
• Limitations(p. 5)
• Major Differences In Vitro(p. 6)
• Types of Tissue Culture(p. 6)
• 2 Biology of Cultured Cells(p. 9)
• The Culture Environment(p. 9)
• Cell Adhesion(p. 9)
• Cell Proliferation(p. 11)
• Differentiation(p. 11)
• Energy Metabolism(p. 13)
• Initiation of the Culture(p. 15)
• Evolution of Cell Lines(p. 15)
• The Development of Continuous Cell Lines(p. 16)
• Origin of Cultured Cells(p. 16)
• 3 Design and Layout(p. 19)
• Planning(p. 19)
• Construction and Services(p. 20)
• Layout(p. 22)
• 4 Equipment(p. 31)
• Requirements of a Tissue Culture Laboratory Essential Equipment(p. 31)
• Beneficial Equipment(p. 40)
• Useful Additional Equipment(p. 46)
• Consumable Items(p. 48)
• 5 Aseptic Technique(p. 51)
• Objectives of Aseptic Technique(p. 51)
• Elements of Aseptic Environment(p. 52)
• Sterile Handling(p. 55)
• Laminar Flow(p. 56)
• Standard Procedure(p. 58)
• Apparatus and Equipment(p. 62)
• 6 Safety(p. 65)
• Risk Assessment(p. 65)
• General Safety(p. 68)
• Fire(p. 72)
• Radiation(p. 72)
• Biohazards(p. 73)
• 7 Culture Vessels(p. 77)
• The Substrate(p. 77)
• Choice of Culture Vessel(p. 78)
• Specialized Systems(p. 83)
• Treated Surfaces(p. 83)
• 8 Media(p. 89)
• Development of Media(p. 89)
• Physicochemical Properties(p. 89)
• Balanced Salt Solutions(p. 93)
• Complete Media(p. 94)
• Serum(p. 99)
• Selection of Medium and Serum(p. 101)
• Other Supplements(p. 103)
• 9 Serum-Free Media(p. 104)
• Disadvantages of Serum(p. 105)
• Advantages of Serum-Free Media(p. 110)
• Disadvantages of Serum-Free Media(p. 111)
• Replacement of Serum(p. 111)
• Selection of Serum-Free Medium(p. 116)
• Development of Serum-Free Medium(p. 117)
• Preparation of Serum-Free Medium(p. 120)
• Conclusions(p. 120)
• 10 Preparation and Sterilization(p. 121)
• Apparatus(p. 121)
• Reagents and Media(p. 130)
• Control, Testing, and Storage of Media(p. 146)
• 11 Primary Culture(p. 149)
• Types of Primary Cell Culture(p. 149)
• Isolation of the Tissue(p. 149)
• Primary Culture(p. 157)
• 12 Cell Lines(p. 177)
• Nomenclature(p. 177)
• Subculture and Propagation(p. 177)
• Immortalization of Cell Lines(p. 180)
• Cell Line Designations(p. 180)
• Selection of Cell Line(p. 181)
• Routine Maintenance(p. 181)
• 13 Cloning and Selection(p. 195)
• Cloning(p. 195)
• Stimulation of Plating Efficiency(p. 198)
• Suspension Cloning(p. 200)
• Isolation of Clones(p. 204)
• Replica Plating(p. 207)
• Selective Inhibitors(p. 207)
• Isolation of Genetic Variants(p. 208)
• Interaction with Susbstrate(p. 211)
• 14 Cell Separation(p. 215)
• Cell Density and Isopyknic Sedimentation(p. 215)
• Antibody-Based Techniques(p. 218)
• Magnetic Sorting(p. 219)
• Cell Size and Sedimentation Velocity(p. 222)
• Centrifugal Elutriation(p. 222)
• Fluorescence-Activated Cell Sorting(p. 223)
• Other Techniques(p. 225)
• Beginner's Approach to Cell Separation(p. 226)
• 15 Characterization(p. 229)
• The Need for Characterization(p. 229)
• Morphology(p. 231)
• Chromosome Analysis(p. 241)
• DNA Content(p. 245)
• RNA and Protein(p. 249)
• Enzyme Activity(p. 251)
• Antigenic Markers(p. 254)
• Differentiation(p. 257)
• Authentication(p. 257)
• 16 Differentiation(p. 259)
• Expression of In Vivo Phenotype(p. 259)
• Stages of Commitment and Differentiation(p. 259)
• Proliferation and Differentiation(p. 260)
• Commitment and Lineage(p. 260)
• Markers of Differentiation(p. 261)
• Induction of Differentiation(p. 262)
• Differentiation and Malignancy(p. 266)
• Practical Aspects(p. 266)
• 17 Transformation(p. 269)
• Role in Cell Line Characterization(p. 269)
• What is Transformation?(p. 269)
• Genetic Instability(p. 269)
• Immortalization(p. 271)
• Aberrant Growth Control(p. 277)
• Tumorigenicity(p. 280)
• 18 Contamination(p. 285)
• Sources of Contamination(p. 285)
• Types of Microbial Contamination(p. 289)
• Monitoring for Contamination(p. 289)
• Eradication of Contamination(p. 294)
• Cross-Contamination(p. 295)
• Conclusions(p. 296)
• 19 Cryopreservation(p. 297)
• Need for Cryopreservation(p. 297)
• Preservation(p. 297)
• Cell Banks(p. 307)
• Transporting Cells(p. 308)
• 20 Quantitation(p. 309)
• Cell Counting(p. 309)
• Cell Weight(p. 314)
• DNA Content(p. 314)
• Protein(p. 314)
• Rates of Synthesis(p. 315)
• Preparation of Samples for Enzyme Assay and Immunoassay(p. 317)
• Cytometry(p. 317)
• Replicate Sampling(p. 318)
• Cell Proliferation(p. 319)
• Plating Efficiency(p. 323)
• Labeling Index(p. 325)
• Cell Cycle Time (Generation Time)(p. 327)
• Cell Migration(p. 328)
• 21 Cytotoxicity(p. 329)
• Introduction(p. 329)
• In Vitro Limitations(p. 330)
• Nature of the Assay(p. 330)
• Anticancer Drug Screening(p. 340)
• Transformation(p. 340)
• Inflammation(p. 343)
• 22 Specialized Cells(p. 345)
• Epithelial Cells(p. 345)
• Mensenchymal Cells(p. 364)
• Neuroectodermal Cells(p. 373)
• Hematopoietic Cells(p. 380)
• Gonads(p. 384)
• 23 Tumor Cells(p. 385)
• Sampling(p. 386)
• Disaggregation(p. 387)
• Primary Culture(p. 387)
• Characterization(p. 387)
• Development of Cell Lines(p. 388)
• Selective Culture(p. 388)
• Specific Tumor Types(p. 392)
• 24 Organotypic Culture(p. 395)
• Cell Interaction and Phenotypic Expression(p. 395)
• Organ Culture(p. 396)
• Histotypic Culture(p. 399)
• Filter-Well Inserts(p. 403)
• Cultures of Neuronal Aggregates(p. 405)
• Organotypic Culture(p. 406)
• 25 Scale-Up(p. 407)
• Scale-up in Suspension(p. 407)
• Scale-up in Monolayer(p. 412)
• 26 Specialized Techniques(p. 423)
• Lymphocyte Preparation(p. 423)
• Autoradiography(p. 424)
• Time-Lapse Recording(p. 429)
• Confocal Microscopy(p. 431)
• Cell Synchrony(p. 431)
• Culture of Amniocytes(p. 432)
• Culture of Cells from Poikilotherms(p. 437)
• 27 Molecular Techniques(p. 443)
• Molecular Biology in Cell Culture(p. 443)
• In Situ Molecular Hybridization(p. 443)
• Somatic Cell Fusion(p. 449)
• Production of Monoclonal Antibodies(p. 452)
• DNA Transfer(p. 455)
• 28 Problem Solving(p. 463)
• Slow Cell Growth(p. 463)
• Choice of Medium(p. 465)
• Unstable Reagents(p. 466)
• Purity of Constituents(p. 466)
• Plastics(p. 466)
• Glassware(p. 466)
• Microbial Contamination(p. 467)
• Chemical Contamination(p. 469)
• Primary Culture(p. 469)
• Cloning(p. 470)
• Differentiation(p. 471)
• Feeding(p. 471)
• Subculture(p. 471)
• Cross-Contamination(p. 472)
• Cryopreservation(p. 472)
• Granularity of Cells(p. 473)
• Cell Counting(p. 473)
• Viability(p. 474)
• In Conclusion(p. 475)
• Reagent Appendix(p. 477)
• Trade Index(p. 483)
• Glossary(p. 517)
• References(p. 523)
• Index(p. 565)